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目的观察老年大鼠氯胺酮麻醉后,学习记忆能力的改变,同时应用复方麝香注射液(SX),以观测其对学习记忆变化的影响。方法28只老年大鼠(20月龄)随机分为4组,每组7只:G1:空白组,第1d(D0)生理盐水(NS)2ml/kg ip,第2d(D1)至第6d(D5)无药物处理;G2:D0:氯胺酮(KTM,50mg/ml)2ml/kg ip,从D1开始每日NS2ml/kg持续至D5;G3:D0:KTM2ml/kg ip,从D1开始每日SX2ml/d持续至D5;G4:D0:KTM2ml/kg ip,从D1开始每日SX6ml/d持续至D5;采用Morris水迷宫仪进行行为学检测。结果随着训练次数的增加,各组逃避潜伏期呈逐渐缩短趋势;G2组:各d逃避潜伏期均长于G1组(p<0.05);G3组:D1至D4与G1组差异无显著性意义,于D3时逃避潜伏期明显短于G2组,D5时明显长于G1组(p<0.05);G4组:各d与G1组比较差异均无显著性意义(p>0.05)。撤除水下平台后游过原平台所在区域次数:G2、G3组游过原平台区次数仍明显低于G1组;而G4与G1组差别无显著性意义(p>0.05)。结论老年大鼠KTM腹腔注射麻醉后,学习记忆能力明显下降,持续至麻醉后5d仍未恢复,SX对此种学习记忆能力下降具有一定改善作用,以较大剂量(6ml·kg-1·d-1)效果显著,较小剂量(2ml·kg-1·d-1)SX对氯胺酮麻醉后的记忆能力下无明显改善效果。
Objective To observe the changes of learning and memory abilities after ketamine anesthesia in the aged rats, and to apply the compound Shexiang injection (SX) simultaneously to observe its effect on the changes of learning and memory. Methods Twenty-eight aged rats (20 months old) were randomly divided into 4 groups, 7 in each group: G1: blank group, 1d (D0) normal saline (NS) 2ml/kg ip, 2d (D1) to 6d (D5) No drug treatment; G2: D0: Ketamine (KTM, 50 mg/ml) 2 ml/kg ip, NS2 ml/kg daily from D1 to D5; G3: D0: KTM 2 ml/kg ip, daily starting from D1 SX2ml/d continued until D5; G4:D0: KTM2ml/kg ip, starting from D1, daily SX6ml/d continued to D5; Morris water maze was used for behavioral testing. Results With the increase of the number of training, the escape latency of each group showed a gradual shortening trend. In G2 group, the escape latency of each d was longer than that of G1 group (p<0.05). There was no significant difference in G3 group: D1 to D4 and G1 groups. The escaping latency was significantly shorter at D3 than at G2, significantly longer at D5 than at G1 (p<0.05), and at G4, there was no significant difference between d and G1 (p>0.05). The number of times of swimming through the original platform after removing the underwater platform: the number of times that the G2 and G3 groups swim through the original platform area is still significantly lower than that of the G1 group; while there is no significant difference between the G4 and G1 groups (p>0.05). Conclusion After anesthesia with intraperitoneal injection of KTM in old rats, the learning and memory ability decreased significantly and persisted until 5 days after anesthesia. SX has a certain improvement effect on the decline of learning and memory ability, with a larger dose (6 ml·kg-1·d). -1) The effect was significant. Smaller doses (2 ml·kg-1·d-1) of SX had no significant effect on memory after ketamine anesthesia.