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毛木耳和黑木耳是同属的两个种,但它们的生长发育条件要求不同,由孢子萌发成单核菌丝所需的时间也不同。一般毛木耳孢子萌发的时间短,在马钤薯培养基上,培养温度18～25℃条件下,毛木耳发芽时间约6天,黑木耳发芽时间约8天。这是用肉眼观察,以培养基表面看到小白点为准,实际上镜检小白点往往已有锁状联合,所以上述的天数是偏高的。因此,确定多孢子接种的时间,要略为提前些。正确掌握从接种到萌发成单核菌丝所需的时间,与杂交能否成功有着密切的关系。因为在多孢子杂交中,在同一斜面试管上,有很多孢子发芽成单核菌丝,很快与对应的单核菌丝相遇,通过锁状联合而结合成双核菌丝。实现毛木耳与黑木耳的菌丝联合,两种孢子萌发的时间一定要相近,才能得到预期的效果。
Both the fungus and the black fungus are two species of the same genus, but their growth and development conditions require different spore germination into mononuclear hyphae in different time. In general, the spore germination time of A. furcifera is short. Under the condition of culture temperature 18-25 ℃, the germination time of the gerbera is about 6 days and the germination time of the black fungus is about 8 days. This is observed with the naked eye, to see the white spots on the surface of the medium prevail, in fact, microscopic examination of white spots often have lock-like joint, so the above number of days is high. Therefore, to determine the time of inoculation of sporozoites, to be slightly earlier. Correctly grasp the time required from inoculation to germination into mononuclear hyphae, and the success of the hybridization has a close relationship. Because in spore hybridization, many spores germinate into mononuclear hyphae on the same beveled tube, quickly meet the corresponding mononuclear hyphae, and bind to the binuclear hyphae via the lock-like combination. To achieve the joint fungus Auricularia aurora and black fungus, the two spores germination time must be similar in order to get the desired effect.