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目的:建立一种阿达帕林脂质体中药物含量的测定方法。方法:采用一阶导数紫外分光光度法,测定波长279 nm处的一阶导数峰-零谷值与阿达帕林脂质体中药物含量的线性关系。结果:在279 nm处一阶导数峰-零谷值可有效消除辅料磷脂对阿达帕林测定的干扰,阿达帕林在6.12~36.72μg.mL-1范围内,浓度与零谷值之间存在良好的线性关系,r=0.999 7;平均回收率为99.42%,RSD为0.72%(n=9);日内及日间精密度的RSD均小于1%(n=5)。经配对t检验,该方法的测定结果与用HPLC法测定结果没有显著性差异(P>0.05)。结论:该方法简便、快速、结果准确,适于测定阿达帕林脂质体中药物含量。
Objective: To establish a method for the determination of drug content in adapalene liposomes. Methods: The first derivative UV spectrophotometry was used to determine the linear relationship between the first derivative peak at zero wavelength at 279 nm and the drug content of adapalene liposomes. Results: The first derivative peak at zero point at 279 nm could effectively eliminate the interference of adaptative phospholipid assay to adapalene. There was a range of 6.12-36.72 μg.mL-1 for adapalene between concentration and zero-valley (R = 0.999 7). The average recovery was 99.42% and the RSD was 0.72% (n = 9). The intra-day and inter-day RSDs were all less than 1% (n = 5). By paired t test, the results of this method were not significantly different from those determined by HPLC (P> 0.05). Conclusion: The method is simple, rapid, accurate and suitable for the determination of drug content in adapalene liposomes.