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研究药物没食子鞣质及其有效提取物对牙菌斑生物膜形态结构和活力的影响,了解常态生物膜形成过程中生物膜活力的变化,以及药物作用下生物膜形态结构和活力的变化,探讨药物对牙菌斑生物膜的作用机制。采用倒置荧光显微镜结合特异荧光染料标记常态牙菌斑生物膜生长过程中死菌和活菌变化,及药物处理后牙菌斑生物膜中死菌和活菌的构成,观察常态牙菌斑生物膜形成过程中生物膜结构及活力的变化以及药物作用下生物膜形态结构和活力的变化;研究和评估常态牙菌斑生物膜的形态结构和细菌活力及实验药物对牙菌斑生物膜结构和细菌活性的影响。结果显示,从早期细菌定植粘附到形成成熟生物膜结构的各个过程中都存在一定数量的死菌。24h常态生物膜结构中活菌面积占主导地位,有少量的死菌存在,细菌互相紧密粘附在一起,生物膜结构清晰,其间有各管道结构。0.05%洗必泰24h细菌生物膜作用后,细菌总量明显下降,残存滞留的细菌基本为死菌。0.023%的氟化钠(NaF)作用细菌生物膜后,生物膜结构发生了明显的变化,细菌总量明显减少,但是存在的细菌以活菌为主。经过4mg/mL的没食子鞣质及其有效提取物作用后,生物膜中各管道结构模糊不清,细菌变得散开,生物膜细菌总量减少,与24h常态生物膜比,有显著差异(P<0.05),生物膜结构密度下降,其中有大量的死菌和部分活菌存在。实验组活菌百分比与阴性对照组活菌百分比相比有显著差异(P<0.05)。由此得出结论,实验药物对牙菌斑生物膜的作用一方面是对生物膜中细菌的杀灭,另一方面是改变牙菌斑生物膜的形态结构,降低生物膜密度及调整生物膜内部死活菌的比例。
To study the effect of the drug tannin and its effective extract on the morphology and viability of the biofilm and to understand the changes of the biofilm activity during the formation of the biofilm and the changes of the biofilm morphology and viability under the action of the drug Mechanism of action of drugs on plaque biofilm. Inverted fluorescence microscopy combined with specific fluorescent dye labeled placental biofilm in the growth process of dead bacteria and viable bacteria changes, and after treatment of dental plaque biofilm dead bacteria and viable components, to observe the normal plaque biofilm Changes in biofilm structure and vitality during formation, and changes in biofilm morphology and viability under the action of drugs. The morphological and bacterial vitality of experimental biofilms and the effect of experimental drugs on the biofilm structure and bacteria Effect of activity. The results show that there is a certain amount of dead bacteria from the early bacterial colonization to the formation of mature biofilm structures. 24h normal biofilm structure in the dominant living area, a small amount of dead bacteria exist, closely adhered to each other bacteria, the biofilm structure is clear, during which the pipeline structure. 0.05% chlorhexidine 24h bacterial biofilm effect, the total bacteria decreased significantly, the remaining bacteria remained basically dead bacteria. When 0.023% sodium fluoride (NaF) acts on the bacterial biofilm, the biofilm structure changes significantly, the total amount of bacteria is significantly reduced, but the bacteria present is mainly viable bacteria. After treated with 4 mg / mL Galla chinensis and its effective extract, the biofilm structure in each tube was unclear, the bacteria became diffused and the total amount of bacteria in the biofilm decreased. Compared with the normal biofilm at 24h, there was a significant difference P <0.05), the density of biofilm decreased, a large number of dead bacteria and some viable bacteria existed. The percentage of viable cells in experimental group was significantly different from the percentage of viable cells in negative control group (P <0.05). It concluded that the role of experimental drugs on the plaque biofilm on the one hand the killing of bacteria in the biofilm, on the other hand is to change the morphology of the plaque biofilm, reduce the density of biofilm and adjust the biofilm The proportion of live bacteria inside.