Dear Editor,CRISPR gene-editing technology has successfully generated targeted mutations in rice and many other plant species (Ma et al.,2015).Assessment of heritability and phenotypic stability of CRISPR-edited plants requires the elimination of the CRISPR construct.The presence of the CRISPR construct makes it difficult to distinguish the mutations transmitted from the previous generation from newly generated mutations by the CRISPR construct at the current generation.The existence of the CRISPR construct also greatly increases the risk of off-target effects,making phenotypic stability a conce.Moreover,removal of the transgenes is likely a prerequisite for gaining regulatory approval of CRISPR-edited crops for commercial applications.Development of an efficient and easy-to-perform technology for the removal of the CRISPR construct after DNA modifications have been completed will have a major impact on using gene-editing technology for crop improvement.