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目的 观察脂膜微囊向鼠脑损伤区聚集的趋向性 ;研究其时相分布及区域分布规律 ;探讨其作用机理。方法 建立射频脑损伤动物模型 ,静脉注射脂膜微囊混悬液 ,荧光素及油红 O染色 ,观察并计数脑切片中脂膜微囊 ,对切片进行双重免疫组化染色 ,计数增生细胞核抗原 (PCNA)和胶原纤维酸性蛋白 (GFAP)阳性细胞。结果 发现脂膜微囊聚集在脑损伤区周围 2mm的范围内 ,伤后 10天出现密度高峰 ,而PCNA、GFAP表达阳性细胞在伤后 48小时密度达高峰。脂膜微囊在伤后第 1日的聚集数量分别为PCNA和GFAP高峰数量的 42倍和 2 0倍。结论 脂膜微囊对鼠脑损伤区有特殊的亲和力 ,能定向聚集于损伤区周围 ,其机理尚需进一步研究。
Objective To observe the tendency of lipid membrane microencapsulation to rat brain injury area, to study its time phase distribution and regional distribution, and to explore its mechanism. Methods The animal model of radio-induced brain injury was established. The suspension of lipid microcapsules, fluorescein and oil red O were intravenously injected. The lipid microcapsules in the brain slices were observed and counted. The sections were subjected to double immunohistochemical staining for counting of proliferating cell nuclear antigen (PCNA) and collagen fibrillary acidic protein (GFAP) positive cells. The results showed that the lipid microcapsules accumulated in the range of 2mm around the brain injury area. The density peak appeared on the 10th day after injury, while the peak of the density of PCNA and GFAP positive cells 48 hours after the injury. The number of lipid microcapsules accumulated on the first day after injury was 42 times and 20 times of the peak numbers of PCNA and GFAP, respectively. Conclusion Lipid microcapsules have special affinity to rat brain injury area and can be gathered around the damaged area in a targeted manner. The mechanism needs further study.