论文部分内容阅读
用ＢＡＬＢ／ｃ×ＬＡＣＡＦ＿１和ＢＡＬＢ／ｃ×Ｃ＿（５７）Ｆ＿１杂交小鼠与纯系ＢＡＬＢ／ｃ小鼠生产腹水制备单克隆抗体，观察各种小鼠对杂交瘤细胞的耐受性，产生腹水的能力及腹水中蛋白质含量和抗体效价。ＢＡＬＢ／ｃ×ＬＡＣＡＦ＿１鼠的平均腹水产量，雄鼠为ＢＡＬＢ／ｃ鼠的２～３倍，雌鼠为１．４倍左右。对杂交瘤细胞的耐受性，腹水中蛋白质含量及抗体效价与ＢＡＬＢ／ｃ小鼠比较均无显著差异。ＢＡＬＢ／ｃ×Ｃ＿（５７）Ｆ＿１小鼠的腹水产量明显低于ＢＡＬＢ／ｃ小鼠，而且死亡率较高。ＢＡＬＢ／ｃ×ＬＡＣＡＦ＿１小鼠生长快，易于饲养繁殖，价格便宜，腹水产量高，是一种替代ＢＡＬＢ／ｃ小鼠生产单克隆抗体腹水较理想的鼠种。
Monoclonal antibodies were prepared from BALB / c × LACAF_1 and BALB / c × C_ (57) F_1 hybrid mice and pure-line BALB / c mice to observe the tolerance of various mice to hybridoma cells and produce ascites The ability of ascites and protein content and antibody titer. The average ascites production in BALB / c × LACAF_1 mice was 2-3 times that of male BALB / c mice and about 1.4 times that of female mice. To the hybridoma cell tolerance, ascites protein content and antibody titer and BALB / c mice no significant difference. The ascites production in BALB / c × C_ (57) F_1 mice was significantly lower than that in BALB / c mice, and the mortality rate was higher. BALB / c × LACAF_1 mice grow fast, are easy to breeding and breeding, cheap, high yield of ascites, is a kind of alternative BALB / c mouse production of monoclonal antibody ascites ideal murine species.