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目的研究二价金属转运体1(DMT1)参与脉络丛上皮细胞系Z310细胞铅转运的机制。方法采用原子吸收分光光度法检测Z310细胞铅吸收的时间效应。免疫荧光法观察DMT1在Z310细胞内的表达。采用siRNA干涉DMT1表达,荧光实时定量PCR检测DMT1的表达水平。原子吸收分光光度法检测DMT1 siRNA干涉对细胞铅吸收影响。结果醋酸铅暴露后,Z310细胞铅吸收量呈时间-依赖性增高;DMT1在Z310细胞中有较丰富的表达,主要在胞质中呈较均匀的分布。与阴性对照组相比,siRNA干涉后,Z310细胞DMT1 mRNA表达水平下降了74.8%。DMT1siRNA干涉后细胞铅吸收量减少了52.3%(P<0.01)。结论在脉络丛上皮细胞吸收铅的过程中,DMT1起到重要的转运作用。
Objective To study the mechanism of lead transport by divalent metal transporter 1 (DMT1) in choroid plexus epithelial cell line Z310. Methods The time effect of lead absorption in Z310 cells was detected by atomic absorption spectrophotometry. Immunofluorescence method was used to observe the expression of DMT1 in Z310 cells. DMT1 was used to interfere with the expression of DMT1 and fluorescence real-time PCR was used to detect the expression of DMT1. Effect of DMT1 siRNA interference on lead absorption by atomic absorption spectrophotometry. Results After exposure to lead acetate, lead uptake in Z310 cells increased in a time-dependent manner. DMT1 was abundantly expressed in Z310 cells and mainly distributed evenly in the cytoplasm. Compared with the negative control group, the expression level of DMT1 mRNA in Z310 cells decreased by 74.8% after siRNA interference. Lead uptake by DMT1 siRNA decreased by 52.3% (P <0.01). Conclusion DMT1 plays an important role in the absorption of lead in choroid plexus epithelium.