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目的观察不同培养时间和细菌密度对重组人白细胞介素-2(rhIL-2)基因工程菌的收获量和rhIL-2表达量的影响。方法:用40L发酵罐进行了3批发酵试验。结果:工程菌于30℃培养4.5~5h,细菌生长密度(OD600)在1.7~2.0之间。在此条件下,工程菌再经42℃诱导培养4h,菌体的收获量和表达水平都比较理想,其湿重和表达量的均值分别可达6.3g/L(菌液)和31.6%。结论:诱导前不同培养时间和细菌密度能显著影响rhIL-2基因工程菌的产量和rhIL-2的表达率。
Objective To observe the effect of different culture time and bacterial density on the amount of recombinant rhIL-2 genetically engineered bacteria and the amount of rhIL-2 expression. Methods: Three batches of fermentation experiments were carried out in a 40 L fermentor. Results: The engineered bacteria cultured at 30 ℃ 4.5 ~ 5h, bacterial growth density (OD600) between 1.7 ~ 2.0. Under these conditions, the engineered bacteria were further cultured at 42 ℃ for 4h. The harvest and expression levels of the bacterial cells were ideal, and the average values of wet weight and expression reached 6.3g / L (bacterial liquid) and 31 respectively. 6%. Conclusion: Different cultivation time and bacterial density before induction can significantly affect rhIL-2 genetically engineered bacteria production and rhIL-2 expression rate.