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目的观察不同培养时间和细菌密度对重组人白细胞介素-2（rhIL-2）基因工程菌的收获量和rhIL-2表达量的影响。方法：用40L发酵罐进行了3批发酵试验。结果：工程菌于30℃培养4．5～5h，细菌生长密度（OD600）在1．7～2．0之间。在此条件下，工程菌再经42℃诱导培养4h，菌体的收获量和表达水平都比较理想，其湿重和表达量的均值分别可达6．3g／L（菌液）和31．6％。结论：诱导前不同培养时间和细菌密度能显著影响rhIL-2基因工程菌的产量和rhIL-2的表达率。
Objective To observe the effect of different culture time and bacterial density on the amount of recombinant rhIL-2 genetically engineered bacteria and the amount of rhIL-2 expression. Methods: Three batches of fermentation experiments were carried out in a 40 L fermentor. Results: The engineered bacteria cultured at 30 ℃ 4.5 ~ 5h, bacterial growth density (OD600) between 1.7 ~ 2.0. Under these conditions, the engineered bacteria were further cultured at 42 ℃ for 4h. The harvest and expression levels of the bacterial cells were ideal, and the average values of wet weight and expression reached 6.3g / L (bacterial liquid) and 31 respectively. 6%. Conclusion: Different cultivation time and bacterial density before induction can significantly affect rhIL-2 genetically engineered bacteria production and rhIL-2 expression rate.