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目的探讨外周血 CD_4~+CD_(25)~+调节性 T 淋巴细胞(CD_4~+CD_(25)~+Tr 细胞)的比例和功能在原因不明复发性流产(URSA)中的作用。方法采用双荧光标记流式细胞分析技术检测15例 URSA 患者(流产组)、15例正常非妊娠妇女(正常非孕组)和13例正常妊娠妇女(正常妊娠组)外周血中CD_4~+CD_(25)~+Tr 细胞的比例。采用磁珠分选方法从3组妇女外周血淋巴细胞中分离 CD_4~+CD_(25)~+Tr 细胞和CD_4~+CD_(25)~-T 淋巴细胞,通过体外增殖抑制实验计算抑制率来评价 CD_4~+CD_(25)~+Tr 细胞对 CD_4~+CD_(25)~-T 淋巴细胞的抑制功能。结果流产组妇女外周血中 CD_4~+CD_(25)~+Tr 细胞的比例为(6.9±1.8)%,明显低于正常非孕组的(10.8±1.1)%(P<0.05)和正常妊娠组的(11.2±1.4)%(P<0.01)。流产组妇女CD_4~+CD_(25)~+Tr 细胞的抑制率为(75±6)%,明显低于正常非孕组的(89±4)%(P<0.05)和正常妊娠组的(90±4)%(P<0.01)。正常非孕组与正常妊娠组妇女 CD_4~+CD_(25)~+Tr 细胞的比例和抑制率比较,差异均无统计学意义(P>0.05)。结论 URSA 的发生可能与外周血 CD_4~+CD_(25)~+Tr 细胞的比例下降和功能降低有关。
Objective To investigate the role of the proportion and function of CD_4 ~ + CD_ (25) ~ + regulatory T lymphocytes (CD_4 ~ + CD_ (25) ~ + Tr cells) in unexplained recurrent spontaneous abortion (URSA) Methods Double fluorescent labeling flow cytometry was used to detect CD_4 ~ + CD_4 in peripheral blood of 15 URSA patients (abortion group), 15 normal non-pregnant women (normal non-pregnant group) and 13 normal pregnant women (normal pregnancy group) (25) ~ + Tr cells. The CD_4 ~ + CD_ (25) ~ + Tr cells and CD_4 ~ + CD_ (25) ~ -T lymphocytes were isolated from the peripheral blood lymphocytes of three groups by magnetic bead sorting. The inhibition rates were calculated by in vitro proliferation inhibition experiments To evaluate the inhibitory effect of CD_4 ~ + CD_ (25) ~ + Tr cells on CD_4 ~ + CD_ (25) ~ -T lymphocytes. Results The proportion of CD_4 ~ + CD_ (25) ~ + Tr cells in peripheral blood of abortion group was (6.9 ± 1.8)%, which was significantly lower than that of normal non-pregnant group (10.8 ± 1.1)% and normal pregnancy (11.2 ± 1.4)% of the group (P <0.01). The inhibition rate of CD_4 ~ + CD_ (25) ~ + Tr cells in abortion group was (75 ± 6)%, which was significantly lower than that in normal non-pregnant group (89 ± 4)% (P < 90 ± 4)% (P <0.01). The percentage of CD_4 ~ + CD_ (25) ~ + Tr cells in normal non-pregnant group and normal pregnant women were not significantly different (P> 0.05). Conclusion The occurrence of URSA may be related to the decrease of the proportion of CD_4 ~ + CD_ (25) ~ + Tr cells in peripheral blood and the decrease of function.