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为比较基于海洋石油降解菌群DC10的菌液制剂(ZL 201010223463.6)与干粉制剂在模拟溢油岸滩修复中试实验中的石油降解效果。通过监测生物修复过程中的基于降解指数n-C17/Pr(正十七烷/姥鲛烷)和n-C18/Ph(正十八烷/植烷)计算的石油烃降解率变化,以及海水中石油烃和氮、磷的浓度变化,综合评价菌剂的岸滩修复效果。结果表明,28 d后添加了菌液制剂处理池的石油烃降解率分别为43.47%和45.07%,比自然风化提高了24.70%和25.40%;而添加了干粉制剂处理池的石油烃降解率分别为40.06%和42.36%,比自然风化提高了21.29%和23.69%。在模拟溢油岸滩修复实验中,干粉制剂的降解效果略低于菌液制剂,但干粉制剂可长期保存,且运输方便,因此干粉制剂比菌液制剂在岸滩溢油修复领域具有更好的应用前景。
In order to compare the degradation effect of petroleum based on DC10 bacterial strain (ZL 201010223463.6) and dry powder formulations in simulated oil spill shore rehabilitation pilot experiment. By monitoring changes in petroleum hydrocarbon degradation rates based on the degradation index n-C17 / Pr (n-heptadecane / pristane) and n-C18 / Ph (n-octadecane / phytane) during bioremediation, CNPC hydrocarbon and nitrogen, phosphorus concentration changes, a comprehensive evaluation of bacterial agents shoreline repair effect. The results showed that the petroleum hydrocarbon degradation rate of the treatment tank added with the bacterial liquid preparation was 43.47% and 45.07% after 28 days, which was 24.70% and 25.40% higher than that of the natural weathering treatment respectively. The degradation rates of petroleum hydrocarbons were 40.06% and 42.36% respectively, 21.29% and 23.69% more than natural weathering. In simulating oil spill shore repair experiments, the degradation efficiency of dry powder formulations was slightly lower than that of bacterial liquid formulations, but the dry powder formulations could be stored for long periods and were easily transported, so the dry powder formulations had a better performance than the bacterial liquid formulations in the field of beach oil spill repair The application prospects.