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从短芒大麦的幼穗、幼胚和成熟胚诱导出愈伤组织，建立了胚性悬浮细胞系，再以悬浮培养细胞为材料，结合诱变剂甲基磺酸乙酯处理，在含盐培养基上筛选，得到耐１．２％ＮａＣｌ的耐盐变异体，将耐盐系转入无盐培养基中培养５代，再转入含盐培养基中，仍具有耐盐性。耐盐系的生理生化分析表明，愈伤组织的耐盐极限在１．５％ＮａＣｌ，耐１．２％ＮａＣｌ的愈伤组织的游离脯氨酸含量为对照的６．６８倍，说明脯氨酸参与了植物组织对盐胁迫的调节。相对电导率比对照小２９．９２％，说明耐盐愈伤组织的膜透性比非耐盐愈伤组织小，证实其耐盐力强。耐盐愈伤组织经过分化，获得少量耐盐植株，经耐盐鉴定，耐盐浓度可达４．０４％。
The callus was induced from young ears, immature embryos and mature embryos of Brassica juncea. An embryogenic suspension cell line was established. The suspension culture cells were used as materials, combined with the mutagen, methyl methanesulfonate, The medium was screened on the medium to obtain the salt tolerant variant resistant to 1.2% NaCl. The salt tolerant strain was transferred to the salt-free medium and cultured for 5 generations, and then transferred to the salt-containing medium, still having salt tolerance. Physiological and biochemical analysis of salt-tolerant lines showed that the salt tolerance of callus in 1.5% NaCl, 1.2% NaCl resistant callus free proline 6.68 times the control, indicating that proline Acid participates in the regulation of plant stress on salt stress. The relative electrical conductivity was 29.92% less than that of the control, indicating that the membrane permeability of salt-tolerant callus was smaller than that of non-salt-tolerant callus and its salt tolerance was strong. Salt-tolerant callus after differentiation, access to a small amount of salt-tolerant plants, salt-tolerant identification, salt tolerance up to 4.04%.