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目的:观察大蒜素对Al Cl3致痴呆大鼠脑部衰老的影响以及对脑和血清内蛋白、SOD(超氧化物歧化酶)、ACh E(乙酰胆碱酯酶)含量的影响。方法:取40只雌性SD大鼠,随机分为5组(n=8):空白对照(C组)、模型对照组(N组)、大蒜素高剂量组(H组,40 mg/kg)、大蒜素中剂量组(M组,20 mg/kg)、大蒜素低剂量组(L组,10 mg/kg)。用药组和模型对照组,皮下注射NaNO_2(90 mg/kg),灌胃AlCl_3(800 mg/kg),空白对照组皮下注射同体积生理盐水,灌胃同体积生理盐水做对照,持续30 d。大蒜素高中低剂量组灌胃不同剂量的大蒜素,空白对照组和模型对照组灌胃同体积的生理盐水做对照,持续30 d。实验结束后,测定小鼠的学习记忆能力,测定脑组织和血清中乙酰胆碱酯酶活性、超氧化物歧化酶的活性以及蛋白的含量。结果:与模型组相比,大蒜素组动物逃避潜伏期明显缩短,并能显著增加脑内蛋白、SOD的含量,AChE的含量明显降低。结论:大蒜素能提高记忆再现障碍大鼠的学习记忆能力,其机制可能与促进脑内蛋白和SOD的合成有关。
Objective: To observe the effect of allicin on brain aging in Al Cl3-induced dementia rats and its effect on the content of protein, SOD and ACh E (acetylcholinesterase) in brain and serum. Methods: Forty female SD rats were randomly divided into five groups (n = 8): blank control group (C), model control group (N), allicin high dose group (H group, 40 mg / , Allicin middle dose group (M group, 20 mg / kg), allicin low dose group (L group, 10 mg / kg). The model group and the control group were injected subcutaneously with NaNO_2 (90 mg / kg) and AlCl_3 (800 mg / kg). The blank control group was injected subcutaneously with the same volume of normal saline and the same volume of normal saline was given to the control group for 30 days. Allicin high and low dose groups were given different doses of allicin, the blank control group and the model control group were given the same volume of saline as control for 30 d. After the experiment, the learning and memory abilities of mice were determined, and the activities of acetylcholinesterase, superoxide dismutase and protein in brain tissue and serum were determined. Results: Compared with the model group, the allicin significantly shortened the escape latency and significantly increased the contents of brain protein and SOD, while the AChE content was significantly decreased. CONCLUSION: Allicin can improve the learning and memory abilities of rats with memory impairment. The mechanism may be related to the promotion of brain protein and SOD synthesis.