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目的探讨肿瘤坏死因子抗膀胱癌细胞作用的影响因素。方法将肿瘤坏死因子α(TNF-α)和不同浓度的丝裂霉素(MC)、顺铂(CDDP)以及干扰素α(IFN-α)作用于体外培养的膀胱癌细胞系BIU-87,用噻唑蓝(MTT)法测定细胞毒效果。结果 TNF-α对BIU-87细胞有明显的浓度依赖性抑制作用,MC(2.5～5.0mg/L)或IFN-α(2500U-5000U/ml)与TNF-α(10000U/ml)作用于细胞后的细胞活力及增殖明显低于单用TNF-α、IFN-α或MC实验组。结论 TNF-α与IFN-α或MC对膀胱癌细胞的抑制有协同作用。
Objective To investigate the effect of tumor necrosis factor on bladder cancer cells. Methods Tumor necrosis factor α (TNF-α) and different concentrations of mitomycin (MC), cisplatin (CDDP) and interferon α (IFN- Cytotoxicity was measured by MTT method. Results TNF-αinduced the proliferation of BIU-87 cells in a concentration-dependent manner. MC (2.5-5.0mg / L) or IFN-α (2500-5000U / ml) and TNF- After the cell viability and proliferation was significantly lower than the single use of TNF-α, IFN-α or MC experimental group. Conclusion TNF-α and IFN-α or MC have synergistic effect on the inhibition of bladder cancer cells.