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目的 探讨与 bcl-2翻译起始部位碱基互补的反义 bcl-2寡脱氧核苷酸 ( oligodeoxynucleotide,ODN)对 Bca-CD885细胞内源性 bcl-2表达的阻断作用。方法 以脂质体 Lepofectin为载体 ,一过性转染 2 0 μmol/L 反义及正义 bcl-2 ODN于 Bca CD885细胞中 ,FCM-抗体观测转染后 2 4h、3 6h、48h细胞内 bcl-2基因蛋白表达变化 ,RT-PCR技术检测转染后 2 4h bcl-2 m RNA的表达变化。结果 转染反义 bcl-2 ODN后 2 4h、3 6h、48h Bca CD885细胞内 bcl-2基因蛋白表达与对照组相比都明显下降 ( P<0 .0 5 ) ,而正义组与对照组无差异 ( P>0 .0 5 ) ;正反义组 bcl-2m RNA与对照组相比无明显变化 ( P>0 .0 5 )。结论 反义 bcl-2 ODN能在蛋白翻译水平特异性抑制颊癌细胞内源性 bcl-2蛋白表达
OBJECTIVE: To investigate the effect of antisense bcl-2 oligodeoxynucleotide (ODN) complementary to the translation initiation site of bcl-2 on the endogenous bcl-2 expression in Bca-CD885 cells. Methods Liposomes Lepofectin was used as a vector and transiently transfected with 20 μmol / L antisense and sense bcl-2 ODN in Bca CD885 cells. FCM-antibody was used to observe the expression of bcl-2 at 24 h, 36 h and 48 h after transfection -2 gene protein expression was detected by RT-PCR technology 24h after transfection bcl-2mRNA expression changes. Results Compared with control group, the expression of bcl-2 protein in Bca CD885 cells decreased significantly (P <0.05) at 24 h, 36 h and 48 h after transfection with antisense bcl-2 ODN, while the expression of bcl- There was no difference (P> 0.05). The bcl-2 mRNA in the antisense and antisense groups showed no significant difference compared with the control group (P> 0.05). Conclusion Antisense bcl-2 ODN can specifically inhibit the expression of endogenous bcl-2 protein in buccal carcinoma cells at the level of protein translation