在疟疾基因诊断的研究中,目前所用的DNA探针大多是用放射性同位素~(32)P标记的,~(32)P有辐射能量强和检测敏感度高的优点,但是半衰期短,价格昂贵,有辐射危害和易污染环境等不足之处,难以用于疟疾流行病学现场.用非同位素取代放射性同位素标记探针是疟原虫核酸探针研究中的大趋势,作者首次报道用生物素标记DNA探针检测红内期间日疟原虫.1 材料和方法 含间日疟原虫DNA插入片段的重 In the research of genetic diagnosis of malaria, most of the currently used DNA probes are labeled with radioisotope 32 P, which has the advantages of strong radiation and high detection sensitivity, but has short half-life and high price , There are radiation hazards and easy to pollute the environment and other shortcomings, it is difficult to use in the field of malaria epidemiology.Replacement of radioisotopes with non-isotopes is a general trend in the study of Plasmodium nucleic acid probes, the authors first report with biotin DNA probe for detection of Plasmodium vivax during red-blooming.1 Materials and Methods Methods for the detection of Plasmodium vivax DNA fragment