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纤维结合蛋白(FN)是广泛分布在体内的具有多种功能而免疫原性相同的高分子糖蛋白。主要在体内起作特异性调理作用,并在临床上发现,血浆FN水平在严重感染,创伤、多系统器官衰竭、营养不良时显著降低。因此FN的检测对临床诊断及疗效观察都有重要价值,可做为预后不良的指标。本文采用火箭电泳的方法对154名健康人和42例肾衰,58例肝硬化患者做了检测和观察,情况如下:材料和方法1.FN标准抗原、抗体由北京生物制品研究所提供。2.标准曲线的制备取FN标准抗原(800μg/ml)一支,用蒸馏水稀释为400μg/ml、
Fibronectin (FN) is widely distributed in the body with a variety of functions and immunogenicity of the same high-molecular glycoprotein. Mainly in the body as a special role in conditioning and clinical found that plasma FN levels in severe infections, trauma, multiple system organ failure, malnutrition was significantly reduced. Therefore, the detection of FN has important value in clinical diagnosis and curative effect observation, which can be used as an indicator of poor prognosis. In this paper, the method of rocket electrophoresis on 154 healthy subjects and 42 cases of renal failure, 58 cases of cirrhosis patients were detected and observed as follows: Materials and methods 1.FN standard antigen, antibody provided by the Beijing Institute of Biological Products. 2. Preparation of standard curve Take FN standard antigen (800μg / ml) a, diluted with distilled water to 400μg / ml,