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目的从细胞增殖方面观察OK-432对乳腺癌细胞MCF-7的抑制作用。方法通过MTT比色法、软琼脂克隆形成实验和细胞周期检测观察MCF-7细胞的生长情况;通过流式细胞仪检测MCF-7细胞的增殖细胞核抗原(PC-NA)表达。结果 OK-432能明显降低MCF-7细胞的增殖代谢活性而抑制其增殖。药物处理组具有显著的生长抑制作用(F=3.765,P=0.000)。OK-432组S+G2/M期的细胞比例为(25.53±1.77)%,与空白对照组(39.32±2.56)%相比显著下降(P<0.05)。结论 OK-432能显著抑制MCF-7细胞增殖。
Objective To observe the inhibitory effect of OK-432 on breast cancer cell line MCF-7 in terms of cell proliferation. Methods MTT assay, soft agar colony formation assay and cell cycle assay were used to observe the growth of MCF-7 cells. The expression of proliferating cell nuclear antigen (PCNA) in MCF-7 cells was detected by flow cytometry. Results OK-432 can significantly reduce the proliferation and metabolism of MCF-7 cells and inhibit their proliferation. The drug treatment group had significant growth inhibition (F = 3.765, P = 0.000). The percentage of cells in S + G2 / M phase in OK-432 group was (25.53 ± 1.77)%, which was significantly lower than that in control group (39.32 ± 2.56)% (P <0.05). Conclusion OK-432 can significantly inhibit the proliferation of MCF-7 cells.