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目的与方法：探讨稀土对药用植物细胞组织培养的作用和意义。以药用植物黄连为材料，研究不同浓度的稀土对愈伤组织鲜重增加的影响。将从黄连外植体上诱导出的愈伤组织接种于添加不同浓度稀土Ｙｂ３＋和Ｅｕ３＋的６７Ｖ培养基上。培养一段时间后，精密称量鲜重，研究不同浓度稀土对黄连愈伤组织生长的影响和对转入无稀土培养基中的愈伤组织后续生长的影响。结果：０．１ｍｇ·Ｌ－１Ｙｂ３＋和１ｍｇ·Ｌ－１Ｅｕ３＋促进愈伤组织的生长，高浓度稀土抑制愈伤组织的生长。在不含稀土的培养基上的继代培养中，来源于稀土处理的愈伤组织鲜重增加值均比对照低。结论：低浓度的稀土对黄连愈伤组织的生长具有促进作用，低浓度和高浓度的Ｙｂ３＋和Ｅｕ３＋对黄连愈伤组织后续的继代培养均有不利的影响
Objective and Methods: To investigate the effect and significance of rare earth on the tissue culture of medicinal plants. Taking medicinal plant Coptis chinensis as material, the effects of different concentrations of rare earth on the fresh weight increase of callus were studied. The callus induced from Coptis explants was inoculated on 67 V medium supplemented with different concentrations of rare earth Yb3 + and Eu3 +. After a period of cultivation, the fresh weights were weighed accurately to study the effects of different concentrations of rare earths on the growth of callus and the effects on the subsequent growth of callus transferred to a medium without rare earths. Results: 0.1mg · L-1Yb3 + and 1mg · L-1Eu3 + promoted the growth of callus, and high concentrations of rare earth inhibited the growth of callus. In subcultures on rare earth-free medium, fresh weight callus derived from rare earth treatment was lower than the control. CONCLUSION: Low concentration of rare earth can promote the growth of Coptis chinensis callus. Low concentration and high concentration of Yb3 + and Eu3 + have adverse effect on the subsequent subculture of Coptis chinensis