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以抗旱小麦品种长武134和不抗旱小麦品种郑引1号为试材,采用-1.2MPaPEG6000处理种子,研究不同水分条件下小麦幼芽中蛋白质表达和部分生理特性的变化.聚丙烯酰胺凝胶电泳结果表明:水分胁迫可诱导抗旱品种幼芽产生分子量约39.5kDa和23.0kDa两种新蛋白亚基,不抗旱品种则无新亚基产生;在正常供水条件下,随着生育期延长,两品种中分子量为48.5kDa的蛋白亚基表达量逐渐增强,因其对水分敏感且属于新发现蛋白,初步命名为水敏感蛋白.生理特性测定结果表明,水分胁迫条件下长武134的根芽比和相对含水量均高于郑引1号,而细胞膜相对透性和丙二醛含量则低于郑引1号.
The drought-tolerant wheat variety Changwu 134 and the non-drought-tolerant wheat variety Zhengyin-1 were used as experimental material to study the changes of protein expression and some physiological characteristics of wheat germ under different water conditions with -1.2MPa PEG6000 treatment.The polyacrylamide gel The results of electrophoresis showed that water stress induced two new protein subunits with molecular weight of about 39.5 kDa and 23.0 kDa in drought-resistant wheat germ and no new subunit in drought-resistant variety. Under normal water supply conditions, The molecular weight of 48.5kDa protein subunit expression gradually increased, because of its sensitivity to water and are newly discovered proteins, initially named water-sensitive protein.The physiological characteristics of the measured results show that the water stress conditions root bud ratio of Changwu 134 And relative water content were higher than Zheng cited No. 1, while the relative permeability of plasma membrane and malondialdehyde content is lower than Zheng cited No. 1.