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[目的]对凤尾蕨科植物蜈蚣草的组织培养方法进行研究。[方法]以野外采集的叶芽为外植体,以1/2MS+3%蔗糖+0.7%琼脂为基本培养基,通过激素配比试验,筛选蜈蚣草愈伤组织诱导和继代培养的培养基配方。[结果]从外植体诱导出愈伤组织最佳培养基配方为1/2MS+3%蔗糖+0.7%琼脂+0.5g/LPVP+0.1mg/LKT+0.5mg/L2,4-D;从愈伤组织诱导出GGB和从GGB诱导出幼苗最佳培养基配方为1/2MS+3%蔗糖+0.7%琼脂+0.5g/LPVP+1.0mg/LKT+0.01mg/L2,4-D;另外,使用1/2MS+3%蔗糖+0.7%琼脂+0.5g/LPVP+0.5mg/L2,4-D做继代培养可以使愈伤组织持续增殖。[结论]研究直接使用野外材料组织培养,简化了试验步骤,是一种方便快速的蜈蚣草组培方法。
[Objective] The research aimed to study the tissue culture method of Pteris vittata Pteris vittata. [Method] The leaf buds collected from the field were used as explants. 1 / 2MS + 3% sucrose + 0.7% agar was used as the basic medium. Through the hormone test, the callus induction and subculture medium of Pteris vittata formula. [Result] The optimum medium of callus induced from explant was 1 / 2MS + 3% sucrose + 0.7% agar + 0.5g / LPVP + 0.1mg / LKT + 0.5mg / The callus induction of GGB and GGB induced seedling optimal medium formula is 1 / 2MS + 3% sucrose + 0.7% agar + 0.5g / LPVP + 1.0mg / LKT + 0.01mg / L2,4-D; The callus was continuously propagated using 1 / 2MS + 3% sucrose + 0.7% agar + 0.5g / LPVP + 0.5mg / L 2,4-D for subculture. [Conclusion] The study made the direct use of field materials for tissue culture and simplified the test procedure, which was a convenient and rapid method for tissue culture of centipede grass.