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【目的】明确新疆部分地区籽瓜细菌性果斑病发生情况,分析不同分离物16S~23S rRNA的ITS区序列,确定不同分离物该基因区遗传变异情况。【方法】采集并分离来自阜康、额敏、沙湾等地的籽瓜以及五家渠甜瓜的病原细菌,应用细菌ITS区通用引物对各分离物16S~23S rRNA的ITS区序列进行扩增测序,并在GENEBANK数据库中作序列比对确定所分离病原种类,同时分析该ITS区序列差异。【结果】从四个地区籽瓜或甜瓜病组织上分离获得分离物均为噬酸菌属燕麦种西瓜亚种(Acidovorax avenae subsp.Citrulli),研究获得的7个果斑病菌分离物与国内外已报道7个分离物间ITS区序列同源性达99.5%~100%,该区遗传序列间并无显著差异。【结论】新疆阜康、额敏、沙湾等地籽瓜均有细菌性果斑病发生,参与比对的细菌性果斑菌分离物16S~23S rRNA的ITS区基因序列高度保守,该基因区遗传变异较小。
【Objective】 The objective of this study was to determine the occurrence of bacterial fruit spot on seed and melon in some areas of Xinjiang. The ITS sequences of 16S ~ 23S rRNA of different isolates were analyzed to determine the genetic variation of this isolate in different isolates. 【Method】 The pathogenic bacteria of seed-melon from Fukang, Emin, Shawan, etc. and melon of Wujiaqu were collected and separated. The ITS region of 16S ~ 23S rRNA of each isolate was amplified by universal primer of ITS region , And in the GENEBANK database for sequence alignment to determine the type of pathogen isolated, while analyzing the ITS sequence differences. 【Result】 The results showed that the isolates of Acidovorax avenae subsp. Citrulli isolated from the seed or melon diseased tissues of the four regions were isolated from seven S. sclerotiorum isolates studied at home and abroad It was reported that the homology between the ITS sequences of the seven isolates was 99.5% ~ 100%. There was no significant difference between the genetic sequences in this region. 【Conclusion】 Bacterial fruit spot blight occurred in seed banks of Fukang, Emin, Shawan and other places in Xinjiang. The gene sequences of ITS region of 16S ~ 23S rRNA were highly conserved, Genetic variation is smaller.