目的:建立大鼠血浆中葡萄内酯的检测方法,测定葡萄内酯与大鼠血浆蛋白的结合率。方法:采用平衡透析法研究葡萄内酯在大鼠体内与血浆蛋白结合率。利用醋酸乙酯萃取富集袋内外样品中的葡萄内酯,并用HPLC法测定其浓度,进而求出血浆蛋白结合率。结果:在低、中、高3种浓度下,葡萄内酯的血浆蛋白结合率分别为100.0%、(99.7±0.6)%、(99.1±0.8)%。结论:葡萄内酯溶液与大鼠血浆蛋白属高度结合。 OBJECTIVE: To establish a method for the determination of plasma lactone in rat plasma and determine the binding rate of plasma protein between it and rat. Methods: Balancing dialysis was used to study the protein binding rate of plasma lactone in rats. The ethyl lactate was used to enrich the lactone in and out of the sample, and the concentration of the lactone was determined by HPLC, and then the plasma protein binding rate was calculated. Results: The plasma protein binding rates of grape lactone were 100.0%, 99.7 ± 0.6% and 99.1 ± 0.8% at low, medium and high concentrations respectively. Conclusion: The lactone solution and rat plasma protein are highly bound.