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目的:探讨不同基因型的HPV感染与各级别CIN发病的关系。方法:回顾性分析天津医科大学总医院2007年11月~2009年12月妇科门诊就诊患者中经组织学检查确诊为CIN的患者共284例。HPV基因型的检测采用PCR扩增、基因芯片探针杂交分型检测法。结果:随CIN级别进展,HPV感染率逐渐升高。CINⅠ中主要为LR-HPV感染;CINⅢ中主要为单一高危型HPV-16感染,HPV-58为第二优势感染的基因型。HPV-16感染率在CINⅠ与CINⅡ、CINⅡ与CINⅢ之间差异均具有高度统计学意义(P<0.01)。结论:随CIN病情发展和病变严重,HPV感染率逐渐升高,HPV感染的基因型不同,引起CINⅠ的HPV基因型主要为HPV-11、6,引起CINⅡ的HPV基因型主要为HPV-16、6,引起CINⅢ的HPV基因型主要为HPV-16、58。HPV-16感染率与宫颈上皮内瘤样变的级别之间存在着从量变到质变的关系。
Objective: To investigate the relationship between different genotypes of HPV infection and the pathogenesis of CIN at different levels. Methods: A retrospective analysis of Tianjin Medical University General Hospital from November 2007 to December 2009 gynecological clinic patients histologically confirmed as CIN patients a total of 284 cases. HPV genotypes were detected by PCR amplification, gene chip probe hybridization typing test. Results: With the progress of CIN level, HPV infection rate gradually increased. CIN Ⅰ mainly for LR-HPV infection; CIN Ⅲ is mainly a single high-risk HPV-16 infection, HPV-58 is the second predominant genotype. The infection rate of HPV-16 between CINⅠ and CINⅡ, CINⅡ and CINⅢwas highly statistically significant (P <0.01). CONCLUSIONS: With the development of CIN and the severity of the disease, the HPV infection rate gradually increases. The genotypes of HPV infection are different. The HPV genotype of CINⅠis mainly HPV-11,6. The HPV genotype of CINⅡis mainly HPV-16, 6, causing HPV genotype CIN Ⅲ mainly HPV-16,58. The relationship between HPV-16 infection rate and cervical intraepithelial neoplasia level from quantitative to qualitative change.