【摘 要】
：
目的:提高盾叶薯蓣药材的品质。方法:通过组织培养,对辐射诱变获得的株系,进行了生化指标及薯蓣皂苷元含量分析。结果:经诱变获得的株系SOD、POD比活力、APX活性和可溶性蛋白
【机 构】
：
河南中医学院药学院,中国药科大学中药学院,天津天士力集团研究院,
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目的:提高盾叶薯蓣药材的品质。方法:通过组织培养,对辐射诱变获得的株系,进行了生化指标及薯蓣皂苷元含量分析。结果:经诱变获得的株系SOD、POD比活力、APX活性和可溶性蛋白电泳谱带与对照株系相比差异很大;大部分辐射诱变株系薯蓣皂苷元含量都超过了对照株系。结论:经诱导获得的辐射诱变株系在生理生化指标及有效成分含量方面与对照表现出明显差异,说明辐射诱变可以作为盾叶薯蓣优良品种选育的途径。
Objective: To improve the quality of Dioscorea zingiberensis medicinal materials. Methods: Through the tissue culture, biochemical indicators and diosgenin content were analyzed in the strains obtained by radiation mutagenesis. RESULTS: The SOD, POD specific activity, APX activity and soluble protein electrophoresis bands of the strains obtained by mutagenesis were significantly different from those of the control strains; most of the radiation-induced strains had more diosgenin than the control strains. . Conclusion: The induced radiation-induced strains showed significant differences from the control in terms of physiological and biochemical indexes and active ingredient content, indicating that radiation mutagenesis can be used as a breeding method for superior varieties of Dioscorea zingiberensis.
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