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基于传统的检测包装饮用水中铜绿假单胞菌的方法周期较长、效率较低等缺陷，本研究结合了免疫磁珠分离（immunomagnetic separation，IMS）和环介导等温扩增（loop-mediated isothermal amplification，LAMP）两种技术，实现了对包装饮用水中铜绿假单胞菌的快速检测。采用免疫磁珠分离技术对样品中的铜绿假单胞菌进行富集，提取富集液中的DNA，采用环介导等温扩增技术进行特异性扩增。研究了免疫磁珠分离技术对铜绿假单胞菌及非目标菌的捕获效率，研究了环介导等温扩增技术检测铜绿假单胞菌的灵敏度和特异度。结果表明：免疫磁珠分离对于铜绿假单胞菌的捕获效率超过50%，对非目标菌的捕获效率低于3%。免疫磁珠分离-环介导等温扩增技术检测铜绿假单胞菌具有较好的特异性，灵敏度较高，检测限为3.5CFU/mL，且使铜绿假单胞菌的检验流程得到简化，缩短检测时间。“,”Based on the long cycle and low efficiency of traditional methods for detecting Pseudomonas aeruginosa in packaged drinking water, this study combines immunomagnetic separation (IMS) and loop-mediated isothermal amplification ( Loop-mediated isothermal amplification (LAMP) two technologies have realized the rapid detection of Pseudomonas aeruginosa in packaged drinking water. The immunomagnetic bead separation technology is used to enrich Pseudomonas aeruginosa in the sample, the DNA in the enrichment solution is extracted, and the loop-mediated isothermal amplification technology is used for specific amplification. The capture efficiency of the immunomagnetic bead separation technology for Pseudomonas aeruginosa and non-target bacteria was studied,and the sensitivity and specificity of the loop-mediated isothermal amplification technology for the detection of Pseudomonas aeruginosa were studied. The results show that the capture efficiency of immunomagnetic bead separation for Pseudomonas aeruginosa is more than 50%, and the capture efficiency for non-target bacteria is less than 3%. The immunomagnetic bead separation-loop-mediated isothermal amplification technology for the detection of Pseudomonas aeruginosa has good specificity, high sensitivity, and a detection limit of 3.5CFU/mL, which simplifies the detection process of Pseudomonas aeruginosa. Shorten the detection time.