论文部分内容阅读
目的探讨不同时间段联合使用铜绿假单胞菌注射液及匹多莫德片治疗后复发性生殖器疱疹(RGH)豚鼠模型的免疫状况,借以间接揭示人类RGH患者长期间隙性服用免疫调节剂的疗效。方法用HSV-2 Sav感染已排除HSV感染的雌性中华彩豚,造成RGH豚鼠模型后,用双抗体夹心酶联免疫法(ELISA)分别先后测出不同用药时间段(3个月,6个月,12个月)、RGH豚鼠模型组(未给药)及空白对照组的豚鼠血清IL-5,10,13及血清IFN-γ的浓度(pg/m L),再分别进行比较。结果 RGH豚鼠模型组IL-5,10及IL-13的浓度均显著高于空白对照组,不同用药时间段豚鼠的IL-5,10及IL-13浓度均低于RGH豚鼠模型组,差异有统计学意义(P<0.01),而RGH豚鼠模型组IFN-γ的浓度显著低于空白对照组,不同用药时间段豚鼠的IFN-γ浓度均高于RGH豚鼠模型组,相比较差异有统计学意义(P<0.05)。结论不同时间段联合使用铜绿假单胞菌注射液及匹多莫德片后可纠正RGH豚鼠模型机体细胞免疫与体液免疫的分化状态。
Objective To investigate the immune status of guinea pigs with recurrent genital herpes (RGH) treated with Pseudomonas aeruginosa injection and Pidotimod tablets in different time periods to indirectly reveal the long-term clearance of immunomodulatory agents in human RGH patients . Methods HSV-2 Sav was used to infect HSC-infected female chinchillas. After RGH-induced guinea pig model was established, different dosage periods (3 months and 6 months) were measured by ELISA, respectively , 12 months), guinea pig serum IL-5,10,13 and serum IFN-γconcentration (pg / m L) in RGH guinea pig model group (untreated) and blank control group respectively. Results The concentrations of IL-5, 10 and IL-13 in RGH group were significantly higher than those in blank control group. The concentrations of IL-5, 10 and IL-13 in guinea pigs in different treatment groups were lower than that in RGH guinea pig model group (P <0.01), while the concentration of IFN-γin RGH guinea pig model group was significantly lower than that of the blank control group. The IFN-γconcentration of guinea pigs in different treatment groups were higher than that in RGH guinea pig model group, the difference was statistically significant Significance (P <0.05). Conclusion The combined use of Pseudomonas aeruginosa injection and Pidotimod tablets at different time points can correct the cellular and humoral immune differentiation of RGH guinea pig model.