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目的:分析I期压疮大鼠受压组织皮肤血流灌注量变化与组织中超氧化物歧化酶(SOD)、丙二醛(MDA)水平影响。方法:选择SD级大鼠40只,随机分为低压组与高压组各20例。低压力组大鼠组织给予局部组织承压22kPa;高压力组给予组织承压28kPa;建模成功后,采用激光多普勒血流仪检测2组大鼠局部皮肤阻断后反应性充血微血流变化最大值(PROH max)与最小值(PROH min),计算微血流最大值与最小值变化时间差(Tp)。采用硫代巴比妥酸法测定丙二醛(MDA),化学发光法测定超氧化物歧化酶(SOD)。观察2组大鼠组织中SOD、MDA水平差异。结果:低压组与高压组大鼠组织中PROH max水平[(12.43±2.95)比(19.72±3.26),t=3.310]、PROH min[(7.25±0.76)比(4.27±0.51),t=2.719]、Tp[(8.16±1.17)比(14.90±1.47),t=2.890],2组比较差异有统计学意义(P<0.05)。组织匀浆中MDA[(2.27±0.61)nmol/L比(5.87±0.36)nmol/L,t=3.163]明显下降、SOD[(46.90±5.72)U/mL比(23.19±6.51)U/mL,t=3.042]明显上升,2组比较差异也有统计学意义(P<0.05)。结论:压力不同,I期压疮大鼠受压组织局部皮肤血流量变化存在差异,其局部氧化应激反应水平也存在明显区别。
OBJECTIVE: To analyze the changes of skin perfusion and the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in the tissues of stage I pressure ulcer rats. Methods: Forty SD rats were randomly divided into low pressure group and high pressure group, 20 cases each. The pressure in the low-pressure group was given to the local tissue under pressure of 22 kPa; the high-pressure group was given the pressure of 28 kPa; after successful modeling, the laser-induced Doppler flowmeter was used to measure the reactive oxygen species The maximum change in flow rate (PROH max) and the minimum value (PROH min) were used to calculate the difference between the maximum and minimum changes in microvessel flow (Tp). Malondialdehyde (MDA) was determined by thiobarbituric acid method and superoxide dismutase (SOD) by chemiluminescence method. The difference of SOD and MDA in the two groups was observed. Results: Compared with the control group, the levels of PROH max in the low-pressure group and high-pressure group [(12.43 ± 2.95) vs (19.72 ± 3.26), t = 3.310], PROH min [(7.25 ± 0.76) vs (4.27 ± 0.51) ], Tp (8.16 ± 1.17), (14.90 ± 1.47), t = 2.890, respectively. The difference between the two groups was statistically significant (P <0.05). The MDA (2.27 ± 0.61) nmol / L (5.87 ± 0.36) nmol / L, t = 3.163] significantly decreased in the tissue homogenate compared with the control group (46.90 ± 5.72) U / mL , t = 3.042], and the difference between the two groups was also statistically significant (P <0.05). CONCLUSIONS: Different pressures lead to differences in the changes of local skin blood flow in pressure-bearing tissues in stage I pressure ulcer rats. There is also a significant difference in the local levels of oxidative stress.