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目的探讨N5,N10-亚甲基四氢叶酸还原酶(MTHFR)基因多态性与冠心病的关系。方法对2003年11月至2005年8月贵阳医学院附属医院及贵州市第二人民医院住院的87例冠心病患者(冠心病组)及同期在门诊进行健康体检的73名健康人(对照组),采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测其MTHFRC677T基因多态性。结果对照组与冠心病组MTHFR677位T等位基因的分布频率分别是18.5%,36.1%,病例组MTHFR基因C677T的CT基因型及T等位基因频率显著高于对照组,差异有显著性(P<0.05)。结论MTHFRC677T基因多态性与冠心病密切相关。
Objective To investigate the relationship between N5, N10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and coronary heart disease. Methods Eighty-seven CHD patients (coronary heart disease group) hospitalized from November 2003 to August 2005 in Guiyang Medical College Hospital and Guizhou Second People’s Hospital, and 73 healthy people (control group ). The MTHFRC677T gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The distribution frequencies of MTHFR677 T allele in control group and CHD group were 18.5% and 36.1% respectively. The CT genotype and T allele frequency of MTHFR gene C677T in case group were significantly higher than those in control group (P <0.05) P <0.05). Conclusion MTHFRC677T gene polymorphism is closely related to coronary heart disease.