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1 材料 药物:复方苦参洗液(由苦参、蛇床子、百部、冰片等10余味中药级成),湖南中医学附属结一医院中心实验室提供,每100ml含生药49.2g。洁尔阴,成都恩威制药有限公司生产,每瓶120ml,批号:9707071。菌种:白色念珠菌,从霉菌性阴道炎病人阴道分泌物中分离,试验采用第二代纯培养物。培养基:沙氏液体培养基,自制。动物:新西兰兔60只,均为雌性,体重2.1±0.45kg,由湖南医科大学附二医院实验动物中心提供,动物级别:一级,合格证叫:001号。所有家兔试验前均经阴道涂片检查,有真菌感染者,弃去不用。
1 Materials Drugs: Compound Kushen lotion (made from 10 Chinese herbal medicines such as Sophora flavescens, Cnidium, Baibu, Borneol, etc.), provided by the Central Laboratory of the First Affiliated Hospital of Hunan Traditional Chinese Medicine, containing 49.2g of crude drug per 100ml. Jieer Yin, produced by Chengdu Enwei Pharmaceutical Co., Ltd., 120ml per bottle, batch number: 9707071. Strains: Candida albicans, isolated from the vaginal secretions of patients with fungal vaginitis, using a second-generation pure culture. Culture medium: Sabouraud liquid medium, homemade. Animals: 60 New Zealand rabbits, all female, weighing 2.1±0.45 kg, provided by Experimental Animal Center, Second Affiliated Hospital of Hunan Medical University, animal grade: Level 1, certificate of conformity: No. 001. All rabbits were examined by vaginal smears before the test. Those with fungal infections were discarded.