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目的通过模拟第三腰椎横突周围无菌性炎症的方法,建立第三腰椎横突综合征动物实验模型,于两种不同动物重复用同一造模方法以考察其可靠性。方法将90只SD大鼠随机分为四组;正常组、假手术组、材料实验组及模型组。于造模后4、10、30 d进行组织病理学观察;4、lO、30 d进行全血、前列腺环素、内皮素、5-羟色胺观察;0、l、8、15 d进行机械性压迫痛阈实验;将20只日本大耳白纯种兔随机分为四组;模型组、材料实验组、假手术组及正常组。于造模后4、lO、30 d进行组织病理学观察;4、10、30 d进行全血、前列腺环素、内皮素、5-羟色胺观察;0、1、8、15 d进行机械性压迫痛阈实验。结果本模型可以在第三腰椎横突周围出现明显无菌性炎症、淤血、水肿及后期有瘢痕形成,经组织细菌培养,无细菌感染。模型组炎性递质等指标显著高于材料实验组、假手术组及正常组(P<0.01)。结论本模型可以对第三腰椎横突周围无菌性炎症作出真实反映。
Objective To establish a third animal model of transverse process syndrome of the lumbar vertebra by simulating aseptic inflammation around the transverse process of the third lumbar vertebra. The reliability of the second lumbar vertebra was evaluated using the same modeling method. Methods 90 SD rats were randomly divided into four groups: normal group, sham operation group, experimental group and model group. Histopathological examination was performed on the 4th, 10th, 30th day after model establishment. Whole blood, prostacyclin, endothelin and 5-hydroxytryptamine were observed on the 4th, 10th, and 30th day. Pain threshold experiment; 20 Japanese white big purebred rabbits were randomly divided into four groups; model group, material experimental group, sham operation group and normal group. Histopathological examination was performed 4, 10 and 30 days after modeling. Whole blood, prostacyclin, endothelin and 5-hydroxytryptamine were observed on the 4th, 10th, 30th day. Mechanical compression Pain threshold experiment. Results The model showed obvious aseptic inflammation around the transverse process of the third lumbar vertebrae, congestion, edema and scar formation at the later stage. The tissue culture was performed without bacterial infection. The indexes of inflammatory neurotransmitter in model group were significantly higher than those in experimental group, sham operation group and normal group (P <0.01). Conclusion This model can truly reflect the aseptic inflammation around the transverse process of the third lumbar vertebra.