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目的:通过检测乳腺组织缺氧诱导因子-1α(hypoxia-inducible factor,HIF-1α)、Ki-67和血管内皮生长因子(vas-cular endothelial growth factor,VEGF)的表达情况,探讨HIF-1α对乳腺癌细胞增殖及血管生成的作用机制。方法:采用免疫组化法检测70例乳腺癌组织及其癌旁组织和10例正常乳腺组织中HIF-1α、Ki-67和VEGF的表达情况。结果:HIF-1α在乳腺癌组织中阳性表达率为78.6%(55/70),在癌旁组织中为45.7%(32/70);Ki-67在乳腺癌组织中阳性表达率为88.6%(62/70),在癌旁组织中为11.4%(8/70);VEGF在乳腺癌组织中阳性表达率为65.7%(46/70),在癌旁组织中为34.3%(24/70)。HIF-1α、Ki-67和VEGF在乳腺正常组织中均未见表达,结果表明,三者在癌组织中的表达显著高于癌旁及正常组织,差异均有统计学意义,P值均<0.01;且与临床分期和淋巴结转移情况密切相关,P<0.05。HIF-1α与Ki-67、VEGF在乳腺癌组织中的表达呈中高度相关,关联系数分别为0.65和0.48;且随着HIF-1α表达增强,Ki-67和VEGF的表达也有增强趋势,P<0.01。结论:乳腺癌组织中HIF-1α、Ki-67和VEGF高表达,与组织恶性程度相关,提示HIF-1α、Ki-67和VEGF可能成为预测乳腺癌发生发展、侵袭转移和预后的重要指标;缺氧可能通过某些机制影响乳腺癌细胞增殖及血管生成,HIF-1α可望成为乳腺癌治疗的一个新靶点,值得进一步研究。
Objective: To investigate the expression of hypoxia-inducible factor-1α (HIF-1α), Ki-67 and vas-cular endothelial growth factor (VEGF) in breast tissues, Mechanism of breast cancer cell proliferation and angiogenesis. Methods: Immunohistochemistry was used to detect the expression of HIF-1α, Ki-67 and VEGF in 70 cases of breast cancer tissues and their adjacent tissues and 10 cases of normal breast tissues. Results: The positive rate of HIF-1α expression in breast cancer was 78.6% (55/70) and in paracancerous tissues was 45.7% (32/70). The positive rate of Ki-67 in breast cancer tissues was 88.6% (62/70), and 11.4% (8/70) in adjacent tissues. The positive rate of VEGF in breast cancer tissues was 65.7% (46/70) and in paracancerous tissues (34.3%, 24/70 ). The expression of HIF-1α, Ki-67 and VEGF in normal breast tissues were not observed. The results showed that the expression of HIF-1α, Ki-67 and VEGF were significantly higher than that in adjacent normal tissues and cancerous tissues ; And was closely related to clinical stage and lymph node metastasis, P <0.05. The expressions of HIF-1α and Ki-67 and VEGF in breast cancer tissues were highly correlated with the correlation coefficients of 0.65 and 0.48, respectively. The expressions of Ki-67 and VEGF were also increased with the increase of HIF-1α expression, P <0.01. Conclusion: The high expression of HIF-1α, Ki-67 and VEGF in breast cancer tissues correlates with the degree of malignancy. It is suggested that HIF-1α, Ki-67 and VEGF may be important predictors for the development, invasion, metastasis and prognosis of breast cancer. Hypoxia may affect breast cancer cell proliferation and angiogenesis through some mechanisms. HIF-1α may be a new target for the treatment of breast cancer and warrants further study.