【摘 要】
：
利用基于统计学的实验设计RSM(Responsesurfacemethodology)优化了BurkholderiacepaciaCF-66产新型抗菌活性物质CF66I的发酵培养基组成。首先,用部分重复因子实验对培养基组
【机 构】
：
大连理工大学生物科学与工程系,大连民族学院生命科学学院,大连民族学院生命科学学院,大连民族学院生命科学学院 大连116024,大连116600,大连116600,大连116600
论文部分内容阅读
利用基于统计学的实验设计RSM(Responsesurfacemethodology)优化了BurkholderiacepaciaCF-66产新型抗菌活性物质CF66I的发酵培养基组成。首先,用部分重复因子实验对培养基组分NH4Cl,MgSO4·7H2O,柠檬酸钠及酵母粉浓度对菌株产CF66I的影响进行评价,找出主要影响因子为柠檬酸钠和酵母粉。两者均为正影响,其他组分对CF66I活性的影响不显著。其次用最陡爬坡路径逼近最大响应区域。最后用中心组合设计及响应面分析确定主要影响因子的最佳浓度。菌株在优化培养基中培养较初始培养基CF66I活性提高了约两倍。
The composition of fermentation medium of CF66I, a new antimicrobial active substance produced by Burkholderia cepacia CF-66, was optimized by using RSM (Responsesurfacemethodology). First of all, the influence of the concentration of NH4Cl, MgSO4 · 7H2O, sodium citrate and yeast extract on CF66I production was evaluated by partial repetition factor experiments. The main influencing factors were sodium citrate and yeast extract. Both were positive effects, and other components had no significant effect on CF66I activity. Second, the steepest ascent path approaches the maximum response area. Finally, the center combination design and response surface analysis to determine the optimal concentration of the main impact factor. The strain cultured in optimized medium showed about twice as much CF66I activity as the initial medium.
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