目的　采用体外作用的方式观察氯化镉引起的脾淋巴细胞功能抑制与镉诱导的细胞凋亡之间的关系。方法　在实验中选择了 3 10、6 2 5、12 5 0、2 5 0 0、5 0 0 0 μmol/L共 5个浓度的氯化镉 ,在体外与小鼠淋巴细胞共育不同时间后 ,采用MTT颜色反应法观察淋巴细胞转化 ,用DNA凝胶电泳法及流式细胞仪法检测细胞凋亡。结果　 2 5 0 0和 5 0 0 0 μmol/L氯化镉对小鼠脾淋巴细胞的转化功能产生明显抑制作用 ,并有剂量 反应关系 ,这两个浓度的氯化镉对淋巴细胞转化功能的抑制率 :刀豆蛋白A刺激组分别为 5 0 %和 78% ,脂多糖刺激组分别为 39%和 5 5 % ;同时 12 5 0～ 5 0 0 0 μmol/L氯化镉可诱导脾淋巴细胞发生凋亡 ,流式细胞仪检测结果为 30 %～ 6 0 %。在较高浓度下氯化镉还可以引起细胞存活率下降 ,并且氯化镉诱导细胞凋亡的作用与其抑制脾淋巴细胞功能的作用相比 ,作用时间短、作用浓度低。结论　氯化镉在体外可能以诱导细胞发生凋亡作为其抑制脾淋巴细胞功能的一种机制 Objective To observe the relationship between cadmium chloride-induced inhibition of spleen lymphocyte function and cadmium-induced apoptosis in vitro. Methods A total of 5 concentrations of 3, 10, 65, 125, 250, 250, 500 micromol / L cadmium chloride were selected in the experiment. After co-cultured with mouse lymphocytes in vitro for different time , MTT color reaction was used to observe the transformation of lymphocytes, and the apoptosis was detected by DNA gel electrophoresis and flow cytometry. Results Cadmium chloride at 2550 and 5000 μmol / L significantly inhibited the transformation of mouse splenic lymphocytes and showed a dose-response relationship. The effects of these two concentrations of cadmium chloride on lymphocyte transformation The inhibitory rates were 50% and 78% for concanavalin A stimulation group and 39% and 55% for lipopolysaccharide stimulation group, respectively. In the meantime, cadmium chloride was induced by 125 000 ~ 500 000 μmol / L cadmium chloride Cell apoptosis occurred, flow cytometry results were 30% ~ 60%. Cadmium chloride can also cause cell survival rate decreased at higher concentrations, and the role of cadmium chloride-induced apoptosis compared with its role in inhibiting the function of splenic lymphocytes, the role of time is short, the role of low concentration. Conclusion Cadmium chloride may induce cell apoptosis in vitro as a mechanism of its inhibition of splenic lymphocyte function