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目的确诊1例国内感染田鼠巴贝虫的病例,对患者周边同事进行调查,初步了解田鼠巴贝虫在广西人群中的分布情况。方法提取患者及相关人士血液DNA,共计121份,进行巢式PCR鉴定。以扩增患者18S rRNA基因为分子标记,与GenBank中各巴贝虫序列进行同源性分析,采用最大似然率法(maximum likelihood)构建系统进化树,分析亲缘关系。结果患者感染田鼠巴贝虫病,患者周边同事共40人感染,阳性率为33.06%(40/121);田鼠巴贝虫的系统分类属于感染人的巴贝虫类,与感染牛、犬的巴贝虫亲缘关系较远。结论田鼠巴贝虫在患者周边同事中感染率较高,对接触人员具潜在的感染风险,应加强预防和控制。
Objective To diagnose one case of Babesia vovellae infected by voles in China and investigate the patients’ peripheral colleagues to find out the distribution of Babesia vaporum in Guangxi. Methods A total of 121 blood samples were obtained from patients and related persons for nested PCR. In order to amplify 18S rRNA gene as a molecular marker, homology analysis was carried out with each Babe worm sequence in GenBank. The phylogenetic tree was constructed by using maximum likelihood method to analyze the genetic relationship. Results The patients were infected with Babesiosis voles. A total of 40 patients were infected with peripheral blood of the patients, the positive rate was 33.06% (40/121). The systematic classification of Babesia voles belonged to Babesia, Babes insects kinship far. Conclusions The infection rate of Babesia vaporum in the peripherals of patients is high, which may cause potential infection risk to the contact persons. Therefore, prevention and control should be strengthened.