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目的观察血管生成抑制因子内皮抑素(ES)对视网膜新生血管形成的抑制作用。方法将鼠龄为7d的32只C57BL/6J小鼠随机分为给氧组(12只)、ES治疗组(12只)和对照组(8只)。将给氧组和ES治疗组小鼠置于浓度为(75±5)%高氧环境中生活5d,然后回到正常氧环境中。ES治疗组小鼠在出氧箱后12、36h,一只眼玻璃体腔内注射1μgES,另一只眼注射1μl的磷酸盐缓冲溶液(PBS)作为对照。对照组小鼠生活在正常氧环境中。右旋糖苷-异硫氰酸荧光素(FITC-dextran)视网膜造影整装铺片了解视网膜新生血管改变;计数突破内界膜的内皮细胞数反映视网膜血管增生情况,观察ES对视网膜新生血管形成的抑制作用。结果与给氧组相比,ES治疗组视网膜铺片见视网膜血管分支走行正常,未见明显的无灌注区;ES治疗眼平均每个视网膜切面可见突破内界膜的内皮细胞核数减少,为(5.39±1.52)个,与给氧组[(22.56±2.13)个]比较,差异有统计学意义(P<0.001)。结论ES可有效抑制视网膜新生血管的形成,有望成为治疗血管增生性视网膜病变的新途径。
Objective To observe the inhibitory effect of endostatin (angiogenesis inhibitor) on retinal neovascularization. Methods Thirty-two C57BL / 6J mice (7 days old) were randomly divided into two groups: 12 in the oxygen group, 12 in the ES group and 8 in the control group. Mice in both oxygen-fed and ES-treated groups were exposed to hyperoxia at a concentration of (75 ± 5)% for 5 days and returned to normal oxygen environment. ES treatment mice were injected with 1 μg ES into the vitreous cavity of one eye 12 and 36 h after the oxygen chamber, and 1 μl of phosphate buffered saline (PBS) was injected into the other eye as a control. Control mice live in normal oxygen environment. Dextran-fluorescein isothiocyanate (FITC-dextran) retinal assembly of the entire film to understand the changes of retinal neovascularization; counted to break the number of endothelial cells within the endodermal membrane reflects the status of retinal vascularization, ES observed retinal neovascularization Inhibition. Results Compared with the oxygen-fed group, the retina of ES-treated group showed normal retinal vascular branches and no obvious non-perfusion area. The number of endothelial cells that broke through the inner limiting membrane was reduced in each ES group 5.39 ± 1.52), which was significantly different from that in oxygen-fed group [(22.56 ± 2.13)] (P <0.001). Conclusion ES can effectively inhibit the formation of retinal neovascularization and is expected to become a new approach for the treatment of proliferative retinopathy.