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目的考察益智提取物的制备工艺,建立其提取物的质量控制方法。方法采用先提取挥发油再将残渣进行水提的方法获得益智提取物;建立气相色谱和高效液相色谱控制其提取物质量的方法。结果以超临界CO2流体萃取益智挥发油,然后将药渣加5倍量水煎煮6h再过滤、浓缩得益智水提物。结论采用气相色谱法和高效液相色谱法可以对益智挥发油及其水提物进行质量控制,为更深入的研究益智提取物打下了基础。
OBJECTIVE To study the preparation technology of Puchi extract and establish the quality control method of its extract. Methods The first extraction of volatile oil and then the residue was extracted by water to obtain the puzzle extract; established by gas chromatography and high performance liquid chromatography to control the quality of the extract. Results The supercritical CO2 fluid extraction puzzle volatile oil, and then the dregs plus 5 times the amount of boiling water for 6h and then filtered, concentrated to obtain a beneficial water extract. Conclusion Gas chromatography and high performance liquid chromatography can control the quality of volatile oil and its aqueous extract, which lays a good foundation for the further study of the extract of zoological.