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考察了利用安大略假丝酵母(Candida ontarioensis)静息细胞不对称催化2-氯-1-(3-氯苯基)乙酮合成(R)-2-氯-1-(3-氯苯基)乙醇的转化反应条件.结果表明,当底物浓度为10g/L时,在最适转化条件下反应72h,产物的ee值和产率分别达到99.9%和99.0%.采用4g/L十六烷基三甲基溴化铵对Candida ontarioensis细胞于4℃通透性处理20min后,全细胞的酶活提高2倍以上.当底物浓度提高为30g/L,转化24h后,产物的ee和产率分别达到99.9%和97.5%.该研究为高效制备(R)-2-氯-1-(3-氯苯基)乙醇提供了可行途径,并为生物催化合成芳基手性醇类手性中间体提供了理论指导。
(R) -2-chloro-1- (3-chlorophenyl) ethanone was asymmetrically catalyzed by Candida ontarioensis resting cells. Ethanol conversion reaction conditions.The results showed that when the substrate concentration of 10g / L, under the optimum conditions for 72h reaction, the product of ee value and yield were 99.9% and 99.0% respectively. Using 4g / L cetane The activity of whole-cell enzyme increased more than 2-fold after treated with 4-methyl-ammonium bromide for 20 min at 4 ℃. When the substrate concentration increased to 30 g / L, (99.9%) and 97.5% (97.5%) respectively.This study provides a feasible pathway for the efficient preparation of (R) -2-chloro-1- Intermediates provide theoretical guidance.