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以黑龙江省大豆主栽品种黑河38为母本,以超级结瘤大豆种质资源ZX4为父本,配制杂交组合,在F2及其之后的分离世代,采用水培接种法及蛭石接种法对其进行结瘤性状室内鉴定比较。结果显示,在F2分离世代,水培接种法较蛭石接种法更直观,更便于在室内进行鉴别观察,在对已获得的超级结瘤性状的F3代及其后的各世代进行连续示踪检验时,蛭石接种法更便于操作管理。因此,鉴定种子量有限的F2代个体结瘤性状,宜采用水培接种法,种子量较多的其他世代的示踪检验鉴定,宜采用蛭石接种法。
HeiLongjiang Province, the main cultivars HeiHe 38 as the female parent, super-nodulated soybean germplasm resources ZX4 as the male parent, the preparation of hybrid combinations, F2 and subsequent generations of separation, hydroponics and vermiculite inoculation method Their nodulation traits identified indoor comparison. The results showed that in the F2 segregation generation, hydroponics inoculation method was more visual than vermiculite inoculation method, and it was more convenient to differentiate observation indoors. Continuous tracing was performed in the F3 generation and subsequent generations of the obtained super-nodulation trait When testing, vermiculite inoculation method is more convenient for operation and management. Therefore, the identification of nodules trait limited F2 individuals should adopt hydroponics inoculation method, the other seeds of more generations of tracing test identification, should be used vermiculite inoculation method.