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目的：本研究旨在探索脑干意识中枢损伤对意识状态的影响及其形态学特征，为脑干意识中枢肿瘤的手术与术后治疗提供依据。方法：建立猫脑干意识中枢电凝损毁的动物模型，用意识水平与神经系统功能缺失记分进行神经行为学评价，用Ｅｖａｎｓ蓝和ＨＥ染色做脑干损伤的大体与光镜下观察，用透射电镜和扫描电镜观察脑干损伤的超微结构表现。结果：电凝损毁脑干各部位网状结构可引起不同程度的意识障碍和神经系统功能缺失，其神经系统功能缺失记分均高于假手术组。形态学研究发现，电凝损毁的中心部位以坏死为主，周围则坏死与凋亡并存，周围的微小血管异常是重要的继发性损伤。结论：电凝损毁脑干网状结构可引起意识障碍、神经系统功能缺失、形态学上的广泛异常和迟发性神经元与胶质细胞的死亡。
Objective: The purpose of this study is to explore the impact of brain stem central nervous system injury on the status of consciousness and its morphological characteristics, and to provide the basis for the surgery and postoperative treatment of central brain tumors. Methods: The animal models of cat scratch damage in brain center were established. The neurobehavioral evaluation was made by using the score of consciousness level and neurological deficit. The gross and light microscopic observations of brain stem injury were performed with Evans blue and HE staining. Electron microscopy and scanning electron microscopy of ultrastructural manifestations of brain stem injury. Results: Electrocoagulation damaged the reticular structures of brain stem, which caused different degrees of disturbance of consciousness and nervous system. The scores of nervous system dysfunction were higher than sham-operated group. Morphological studies found that the central part of the destruction of electrocoagulation necrosis, necrosis and apoptosis around the coexist, the surrounding microvascular abnormalities is an important secondary injury. CONCLUSION: Electrocoagulation, which damages the brainstem reticular formation, can cause disturbance of consciousness, loss of nervous system function, extensive morphological abnormalities, and death of late neurons and glial cells.