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随着继代培养次数的增加,燕山红栗(Castanea mollissimac v.’yanshanhong’)组培微枝的生根能力提高。本实验通过测定内源激素水平和酶活性来研究继代培养燕山红栗对不定根生根的作用。对继代2、4、6、8次的微枝诱导生根,通过高效液相色谱法测定内源生长素(IAA)、脱落酸(ABA)、细胞分裂素(CTK)和赤霉素(GA);用紫外分光光度法测定生长素氧化酶(IAAO)、过氧化物酶(POD)和多酚氧化酶(PPO)。微枝的生理特征和继代次数或者生根能力的关系的如下:通过继代培养,内源IAA的含量逐渐上升;ABA、CTK和GA3的含量略有下降。IAA含量和继代次数与生根率高度有正相关;在连续继代过程中,IAA/ABA和IAA/CTK含量比率都剧烈上升,并且与生根率高度相关;IAAO和POD活性与继代次数显著负相关,而PPO活性随着继代次数的增加而提高。图3表1参34。
With the increasing number of subculture, the rooting ability of Castanea mollissimac v.’yanshanhong ’was increased. In this study, the effect of secondary culture of Yanshan Hongliu on adventitious rooting was studied by measuring the level of endogenous hormones and enzyme activity. The rooting of shoots was induced at 2, 4, 6 and 8 times after subculture. The contents of IAA, ABA, CTK and GA were determined by high performance liquid chromatography (HPLC) ); IAAO, POD and PPO were measured by UV spectrophotometry. The relationship between micro-branches physiological characteristics and the number of generations or rooting ability is as follows: The content of endogenous IAA gradually increased by subculture; the contents of ABA, CTK and GA3 decreased slightly. The content of IAA and the number of subcultures were positively correlated with the height of rooting. The content of IAA / ABA and IAA / CTK increased sharply during continuous subculture, and were highly correlated with the rate of rooting. The activities of IAAO and POD were significant Negative correlation, and PPO activity increased with the increase of the number of generations. Figure 3 Table 1 Reference 34.