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目的 :研究白细胞介素类及白三烯类等炎性介质对小鼠腹腔巨噬细胞生成及分泌肿瘤坏死因子α(TNFα)的影响。方法 :用L92 9作为靶细胞的结晶紫染色法测定巨噬细胞培养上清液及胞溶部分TNFα的含量。结果 :重组鼠白细胞介素 1β(rmIL 1β)和重组人白细胞介素 8(rhIL 8)均能促进巨噬细胞产生TNFα ,其中rhIL 8有很好的剂量相关性 ,对巨噬细胞胞溶部分的TNFα无影响 ;重组人白细胞介素 6 (rhIL 6 ) ,白三烯B4 (LTB4 ) ,白三烯C4(LTC4 )及白三烯D4 (LTD4 )均不能促进巨噬细胞生成及分泌TNFα。结论 :rhIL 8和rmIL 1β能促进小鼠腹腔巨噬细胞生成TNFα。rhIL 6 ,LTB4 ,LTC4 和LTD4 对TNFα生成无影响
Objective: To study the effects of inflammatory mediators such as interleukins and leukotrienes on the production and secretion of tumor necrosis factor-α (TNFα) in mouse peritoneal macrophages. Methods : The supernatant of macrophages and the fraction of TNFα in the cytosol were determined by crystal violet staining with L92 9 as target cells. RESULTS: Recombinant murine interleukin 1β (rmIL 1β) and recombinant human interleukin 8 (rhIL 8) both promoted the production of TNFα by macrophages. RhIL 8 has a good dose-response relationship to the macrophage cytolytic fraction. TNFα had no effect; recombinant human interleukin 6 (rhIL 6 ), leukotriene B4 (LTB4), leukotriene C4 (LTC4) and leukotriene D4 (LTD4) failed to promote macrophage production and secretion of TNFα. Conclusion : rhIL 8 and rmIL 1β can promote TNFα production in mouse peritoneal macrophages. rhIL 6, LTB4, LTC4, and LTD4 have no effect on TNFα production