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将甘蓝型油菜的花粉授在同源四倍体小白菜的柱头上时,少数柱头乳突细胞出现胼胝质沉积,杂交的受精过程及胚和胚乳的早期发育基本正常。但与四倍体白菜自交相比,进入花柱的花粉管较少,胚和胚乳的发育进程较缓慢。由于杂种胚发育缓慢,在胚乳细胞发生退化时,多数杂种胚处于晚球形、心形或鱼雷形阶段。随着胚乳细胞的消失,杂种胚停止发育并最终解体。授粉后30d,杂种胚珠大多解体,仅有少数胚珠能发育为成熟可育的种子。因此,在同源四倍体小白菜留种时,必须与甘蓝型油菜进行一定程度的隔离。
When the pollen of Brassica napus was applied to the stigma of the autotetraploid cabbage, a small number of stigma cells appeared callose deposition, and the process of fertilization and the early development of embryo and endosperm were basically normal. However, compared with the tetraploid cabbage inbred, access to the style of the pollen tube less embryo and endosperm development more slowly. Due to the slow development of hybrid embryos, most hybrid embryos are in a late-spherical, heart-shaped or torpedo-shaped stage when endosperm cells degenerate. As the endosperm cells disappear, the hybrid embryos stop developing and eventually disintegrate. On the 30th day after pollination, most of the hybrid ovules disintegrated, and only a few ovules could develop into mature and fertile seeds. Therefore, the autotetraploid Chinese cabbage must be segregated to some extent with Brassica napus.