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为获得正常的甘白杂交后代,本研究利用地方白菜型品种资源雅安黄油菜与常规甘蓝型油菜进行种间杂交,对F0代种子在培养基上利用秋水仙素进行加倍处理,获得了染色体数目在40～58条的混倍体植株,混倍体植株在染色体数目、植株形态、花器官、生殖器官上都明显区别于非加倍杂交植株,并利用加倍提高了甘白杂交F1的自交结实率,获得了正常的F2代植株,说明对油菜种间杂交后代进行加倍处理可以在一定程度上克服由于染色体数目不配对、自交不亲和等现象带来的影响,提高杂交后代自交结实,获得正常的F2代植株。
In order to obtain the normal hybrids, Ganoderma lucidum (Brassica napus L.) and local Brassica campestris L. hybrids were used for interspecific hybridization. The chromosomes of F0 seeds were doubly treated with colchicine on the medium, and the number of chromosomes In the 40 ~ 58 hybrid plants, the diploid plants were significantly different from the non-doubling hybrid plants in chromosome number, plant morphology, floral organs and reproductive organs, Rate, obtained the normal F2 generation plants, indicating that the rape interspecific hybridization progeny doubling treatment can be overcome to some extent due to the number of unpaired chromosomes, self-incompatibility phenomena such as the impact of self-cross to improve the hybrid progenies , Get the normal F2 generation plants.