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目的:探讨依达拉奉对硝普钠诱导的PC12细胞凋亡的影响。方法:体外培养PC12细胞,并分为依达拉奉对硝普钠保护组(含500μmol/L硝普钠和75μmol/L依达拉奉)、硝普钠诱导组(含500μmol/L硝普钠)和对照组。采用MTT法检测细胞的增殖率;流式细胞术检测细胞的凋亡情况;Western-blot检测凋亡抑制蛋白Bcl-2和凋亡促进蛋白Bad的表达。结果:与对照组相比,硝普钠处理的PC12细胞增殖率显著降低,而细胞凋亡率显著升高,细胞内Bcl-2的表达显著减少,而Bad的表显著增加,差异均具有统计学意义(P<0.05);与单纯硝普钠诱导组相比,依达拉奉处理组细的胞增殖率显著增加而细胞凋亡率显著减少,同时Bcl-2的表达显著增加,而Bad的表达明显减少,差异均具有统计学意义(P<0.05)。结论:依达拉奉对硝普钠诱导的PC12细胞凋亡具有抑制作用,可能通过增加Bcl-2的表达并降低Bad的表达发挥抗凋亡作用。
Objective: To investigate the effect of edaravone on apoptosis induced by sodium nitroprusside in PC12 cells. Methods: PC12 cells were cultured in vitro and divided into three groups: edaravone sodium nitroprusside group (500μmol / L sodium nitroprusside and 75μmol / L edaravone), sodium nitroprusside group Sodium) and control group. Cell proliferation was detected by MTT assay. Cell apoptosis was detected by flow cytometry. The expressions of Bcl-2 and Bad protein were detected by Western-blot. Results: Compared with the control group, the proliferation rate of PC12 cells treated with sodium nitroprusside was significantly decreased, while the apoptosis rate was significantly increased. The expression of Bcl-2 was significantly decreased, while the expression of Bad was significantly increased, with statistical differences (P <0.05) .Compared with sodium nitroprusside-induced group, the cell proliferation rate was significantly increased and the apoptosis rate was significantly decreased in edaravone-treated group, while the expression of Bcl-2 was significantly increased The expression was significantly reduced, the difference was statistically significant (P <0.05). Conclusion: Edaravone can inhibit the apoptosis of PC12 cells induced by sodium nitroprusside and may play an anti-apoptotic effect by increasing the expression of Bcl-2 and decreasing the expression of Bad.