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为了解乙肝病毒 (HBV)表面抗原和抗体双阳性患者中病毒的基因型及其HVBS区是否有变异。用放射免疫试剂检测HBsAg阳性样品中的抗 HBs抗体 ,用聚合酶链反应法检测双阳性样品中的HBVDNA ,然后对阳性样品进行克隆和基因序列分析 ,并将所得序列与HBV不同基因型的代表株进行比较分析。结果显示 389例HBsAg阳性样品中有 10例为抗HBs抗体阳性 ;该 10例双阳性样品中有 5例为HBVDNA阳性 ;序列分析显示该 5株HBV均为B基因型 ,其中 4株为adw亚型 ,1株为adr亚型 ;其中有 2株在S区的“a”决定簇的氨基酸发生了变异
In order to understand whether the virus genotype and its HVBS region are mutated in hepatitis B virus (HBV) surface antigen and antibody double positive patients. Anti-HBs antibodies in HBsAg-positive samples were detected by radioimmunoassay, HBVDNA in double-positive samples was detected by polymerase chain reaction, and positive samples were cloned and gene sequence analysis, and the resulting sequences were compared with the representatives of different genotypes of HBV Strains for comparative analysis. The results showed that 10 out of 389 HBsAg positive samples were positive for anti-HBs antibody; 5 of these 10 positive double-positive samples were positive for HBVDNA. The sequence analysis showed that all 5 HBVs were genotype B, of which 4 were adw subtype And 1 strain was adr subtype. Two of them had a mutation in the amino acid of “a” determinant in S region