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采用甲胎蛋白放射免疫分析（ＡＦＰＲＩＡ）及豌豆凝集素亲和交叉免疫电泳自显影法（ＰＳＡ－ＡＦＦ－ＣＩＥＡ），对３０例原发性肝癌及３０例非癌性肝病作ＡＦＰ定量测定及分子异质体比值分析。３０例非癌性肝病中有３例血清ＡＦＰ浓度＞４００μｇ／ｌ，２５例低浓度阳性（３０．７１－３６２．６７μｇ／ｌ）；而３０例原发性肝癌中１０例＜４００μｇ／ｌ（２１．９３－３９１．２５μｇ／ｌ）。提示总量测定难于作出良、恶性肝病的鉴别诊断。但ＡＦＰ分子异质体比值分析，３０例肝癌中豌豆凝集素结合型ＡＦＰ为４１．２６％±１９．４７％，３０例良性肝病为１５．９６％±１２．０９％。二者差异显著（Ｐ＜０．００１）．提示用ＰＳＡ－ＡＦＦ－ＣＩＥＡ检测ＡＦＰ低浓度阳性的良、恶性肝病，具有鉴别诊断价值。
Quantification and characterization of AFP in 30 cases of primary hepatocellular carcinoma and 30 cases of non-cancerous liver disease using alpha-fetoprotein radioimmunoassay (AFPRIA) and pea-lectin affinity immunoelectrophoresis autoradiography (PSA-AFF-CIEA) Heterogeneous ratio analysis. Among 30 cases of non-cancerous liver disease, 3 had serum AFP concentrations >400 μg/l, 25 had low concentrations (30.71-362.67 μg/l), and 30 patients had <400 μg/l in 10 cases of primary liver cancer ( 21.93-391.25 μg/l). It is difficult to make a total differential diagnosis of benign and malignant liver disease. However, the heterogene ratio analysis of AFP moleculars showed that the pea lectin-binding AFP was 41.26%±19.47% in 30 cases of liver cancer, and 15.96%±12.09% in 30 cases of benign liver disease. The difference between the two was significant (P<0.001). The use of PSA-AFF-CIEA to detect AFP low-concentration positive benign and malignant liver disease has differential diagnostic value.