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目的为了深入了解葡萄糖-6-磷酸脱氢酶(G6PD)缺陷症的本质,我们对广西地区56例男性G6PD缺陷症患者从基因水平进行了较为系统的研究。方法应用聚合酶链反应分别扩增包含G6PD基因第Ⅱ、Ⅴ、Ⅵ、Ⅶ、Ⅷ、Ⅸ、Ⅹ、Ⅺ、Ⅻ、外显子的DNA片段,并结合等位基因探针点杂交(ASOdotblot)分别检测G6PD基因cDNA392(G→T)、cDNA487(G→A)、cDNA493(A→G)、cDNA592(C→T)、cDNA835(A→T)、cDNA1311(C→T)、cDNA1360(C→T)、cDNA1376(G→T)、cDNA1388(G→A)突变;结合人工介导的HhaⅠ和天然MboⅡ限制性内切酶图谱技术,分别检测cDNA95(A→G)、cDNA1024(C→T)突变。结果从56例广西籍男性G6PD缺陷者中,共检出6种基因突变类型,分别是cDNA1376(G→T)(25.0%)、cDNA1388(G→A)(16.1%)、cDNA95(A→G)(19.8%)、cDNA592(C→T)(7.1%)、cDNA1024(C→T)(1.8%)、cDNA392(G→T)(1.8%),未知突变28.6%。未发现cDNA1?
Objective To understand the nature of glucose-6-phosphate dehydrogenase (G6PD) deficiency, we conducted a systematic study on the genetic level of 56 male G6PD-deficient patients in Guangxi. Methods DNA fragments containing G6PD gene Ⅱ, Ⅴ, Ⅵ, Ⅶ, Ⅷ, Ⅸ, Ⅹ, Ⅺ, Ⅻ and exons were amplified by polymerase chain reaction (PCR) (G → A), cDNA493 (A → G), cDNA592 (C → T), cDNA835 (A → T), cDNA1311 (C → T), cDNA1360 (A → G) and cDNA1024 (C → T) were detected by using Hha Ⅰ and Mbo Ⅱ restriction enzyme digestion techniques. )mutation. Results Sixty-six cases of G6PD-deficient male Guangxi were detected 6 types of gene mutations: cDNA1376 (G → T) (25.0%), cDNA1388 (G → A) (A → G) (19.8%), cDNA592 (C → T) (7.1%), cDNA1024 , Unknown mutation 28.6%. Did not find cDNA1?