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目的 探讨抗 HEV阴性急性戊型肝炎病人的HEV基因型。方法 应用HEVORF2简并引物进行RT nPCR ,检测 2 2例抗 HEV阴性的急性肝炎病人血清HEVRNA ,应用双脱氧链终止法对部分阳性产物直接测序。结果 2 2例抗 HEV阴性的急性肝炎病人中 ,9例 (40 9% )HEVRNA阳性。对其中 6例阳性产物进行直接测序显示 ,5例为典型中国株 ,与HEV中国株核苷酸和氨基酸序列的同源性分别为98 0 %和 99 0 % ;1例变异较大 ,与HEV中国株、缅甸株、墨西哥株、美国株及猪HEV核苷酸序列的同源性分别为 77 4%、78 1%、74 3%、77 4%、77 1% ,氨基酸序列的同源性分别为 91 0 %、91 0 %、89 0 %、93 0 %、93 0 %。结论 我国除存在典型的中国株HEV感染外 ,还存在HEV变异株感染。
Objective To investigate the HEV genotypes of HEV-negative acute hepatitis E patients. Methods RT nPCR was performed using HEVORF2 degenerate primers. Twenty-two HEV RNAs were detected in HEV-negative acute hepatitis patients, and some of the positive products were sequenced directly by dideoxy chain termination method. Results Among 2 2 anti-HEV-negative acute hepatitis patients, 9 (40.9%) were positive for HEV RNA. Direct sequencing of 6 positive products showed that 5 were typical Chinese strains, with 98.0% and 99.0% homology with the nucleotide and amino acid sequences of HEV strains in China respectively. The homologies of nucleotide sequences of Chinese, Burmese, Mexican, American and pig HEV were 77 4%, 78 1%, 74 3%, 77 4%, 77 1%, respectively. The homology of amino acid sequences Respectively 91 0%, 91 0%, 89 0%, 93 0%, 93 0%. Conclusion In addition to the typical HEV infection in China, HEV variant infection still exists in our country.