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本生烟(Nicotiana benthamiana)与烟草坏死病毒大豆分离物(TNV-A~C)构成的植病互作体系是一种特殊的亲和互作.向本生烟外施一定剂量的抗性诱导剂茉莉酸(JA)、乙烯(ET),和抑制剂丙基没食子酸(PG,JA合成抑制剂)、氯化钴(CoCl_2,ET合成阻断剂)后,用Northern blot,Western blot,半定量RT-PCR等方法,在病毒接种的早、中、晚期,检测TNV及防御基因PR1,PR3,GST1的表达变化.结果显示在诱导剂处理后,TNV表达延迟,病毒含量降低,而抑制剂的作用则相反.防御基因的变化与病毒复制量的变化趋势相同,以PR3最为显著.结果初步表明ET/JA是本生烟-TNV互作系统防御信号途径的关键分子.
Nicotiana benthamiana and Tobacco necrosis virus soybean isolate (TNV-A ~ C) constitute a special interaction between the interaction system.Extract a certain dose of nicotine on the nicotine resistance induction JA, ET, PG and JA inhibitor, and cobalt chloride (CoCl 2, ET synthesis blocker) were synthesized and characterized by Northern blot, Western blot and semi RT-PCR and other methods to detect the expression of TNV and PR1, PR3 and GST1 in the early, middle and late stages of virus inoculation.The results showed that the TNV expression was delayed and the virus content was decreased after the induction treatment, The results showed that ET / JA was the key molecule of defense signaling pathway of Nicotiana benthamiana-TNV interaction system.